怎樣選擇保護柱，但又不影響分離分析？通常，在選擇保護柱之前首先要考慮的是樣品是否清潔，對於大部分分析工作者來說，一隻1cm長的保護柱便能提供充分的保護作用。但是，如果樣品非常不清潔，或工作中發現經常要更換1cm的保護柱，那麼就應該選用2cm或3cmdebaohuzhu，baohuzhuyuechangziransuozhuangtiandeseputianliaojiuyueduo
，zeqibaohuxingnengyuehao


，dangransuizhebaohuzhuchangdudezengjia，yangpindebaoliushijianyexiangyingzengjia，yibanlaishuo，baohuzhudeneijingyufenxisepuzhudeneijingxiangtonghuoxiangdangjike。
baohuzhudetianliaozhuangtianfangshiyehenzhongyao

，muqianyoubomozhuangtianfadebaohuzhu
，shiyongguochengjiandanfangbian，erqiekeyizaishiyanshizhongjinxingganzhuang。danshi，qizuidadequedianshibujingji
，tebieshizhenduizhongguodejutiqingkuang
，yicixingshiyongxiangduifeiyongjiaogao。lingwai，bomozhuangtianfadebaohuzhusuozhuangtiandeseputianliaoyouxian，zhinengtigonghenyouxiandebaohuzuoyong

；不過也因為所裝填的色譜填料較少，保護柱的長度也較短，所以對分析樣品的保留時間的影響也很小。
另一種保護柱結構其實質是縮短了色譜分析柱，設計方式上有直連式/shoujinshihuozhengtishi。zhengtishishejishiyousepuzhudeshengchanchangshangzhijieanzhuangzaisepufenxizhushangde
，bixuyusepufenxizhuyitongdinghuo
，keyifeichangfangbiandeshiyong，danbunengbeixiugai。zhilianshijiegoushejikeyizairenheshihouyousepugongzuozhelaianzhuanglianjie
，keyiyurenhepinpaidesepuzhulianjieshiyong，erqiekeyigenjubutongdeyangpinxuanzeheshidebaohuzhuchangdu。shoujinshibaohuzhucongjiegoushangjiangshiyuzhilianshibaohuzhuyiyang，zhishizailianjieshibuyongjiezhubanshoudenggongju
，zhijieyongshoushangjinjike。lingwaicongbaohuzhujiegoudejiaodukan，baohuzhujiegouyoufenweishifoukeyigenghuanbaohuzhuzhuxin，xianzaidaduoshudebaohuzhujunkeyigenghuanzhuxin，keyijiangdibaohuzhudeshiyongchengben。
daduoshurenshigenjusepufenxizhudetianliaolaixuanzebaohuzhudetianliao，zhengchangqingkuangxiakeyixuanzeyufenxisepuzhuyiyangdeseputianliao。danshi，genjushijidefenxigongzuo，yekebubiyufenxisepuzhudetianliaowanquanpipei。
對於選擇保護柱的原則是：在滿足分離分析要求的前提條件下，盡可能的選擇較短的保護柱，盡可能選擇對分離樣品小一些保留性的填料。