(1)材料
 

瓊脂斜麵培養基
 

經分離培養的平板培養物。
 

(2)方法
 

①在瓊脂斜麵培養基試管上部標明移種的菌名(或編號)、接種日期
、接種者的組別及代號。
 

②zuoshouchichangyouxijundepingbandi，youshouchijiezhonghuan。jiezhonghuanzaihuoyanshangmiejunlengquehou
，zhanqupingbanhuaxianshangfayulianghaodegulijunluo。bapingbanfanghuiyuanchu，lijiyongcishouquxiemianpeiyangjixiechi，yongyoushouxiaozhiyushouzhangjiachimiansai，qingqingzhuandonghoubochumiansai，guankoutongguohuoyanmiejun。
 

③將已沾菌的接種環伸入斜麵培養基的管底部的凝固水

，沿斜麵培養基的表麵從底向上劃一直線，然後再從底部向上劃連續曲折線

；也可不劃直線隻劃曲折線。取出接種環，管口通過火焰滅菌，塞好棉塞


，放試管架上，接種環火焰滅菌後放下。
 

向xiang斜xie麵mian培pei養yang基ji上shang移yi種zhong的de細xi菌jun如ru果guo是shi生sheng長chang在zai斜xie麵mian培pei養yang基ji上shang的de

，或huo是shi生sheng長chang在zai液ye體ti培pei養yang基ji中zhong的de，則ze用yong左zuo手shou同tong時shi斜xie持chi菌jun種zhong管guan和he斜xie麵mian培pei養yang基ji兩liang個ge管guan
，右you手shou無wu名ming指zhi、xiaozhishouzhangtongshibaqijiachiergemiansai。guankoujijiezhonghuanxianjinghuoyanmiejunhou，congjunzhongguanqujunlijiyizhongdaoxiemianshang，saihaomiansai
，jiezhonghuanhuoyanmiejunhoufangxia。ranhoujiangxiemianpeiyangjifang37℃恒溫箱中培養24h。結果斜麵表麵形成菌苔。