由接種環以菌操作沾取少許待分離的材料
，在無菌平板表麵進行平行劃線、shanxinghuaxianhuoqitaxingshidelianxuhuaxian，weishengwuxibaoshuliangjiangsuizhehuaxiancishudezengjiaerjianshao
，bingzhubufensankailai，ruguohuaxianshiyidehua，weishengwunengyiyifensan，jingpeiyanghou，kezaipingbanbiaomiandedaodanjunluo。