細胞培養基配好後，要先抽取少許放入培養瓶內在37℃培養箱內放置24~48h，已(yi)檢(jian)測(ce)培(pei)養(yang)液(ye)是(shi)否(fou)有(you)汙(wu)染(ran)，然(ran)後(hou)方(fang)可(ke)用(yong)於(yu)實(shi)驗(yan)。配(pei)置(zhi)培(pei)養(yang)基(ji)所(suo)需(xu)的(de)血(xue)清(qing)要(yao)合(he)格(ge)並(bing)且(qie)保(bao)持(chi)穩(wen)定(ding)。一(yi)個(ge)批(pi)號(hao)試(shi)用(yong)效(xiao)果(guo)良(liang)好(hao)後(hou)，就(jiu)可(ke)一(yi)次(ci)購(gou)入(ru)較(jiao)多(duo)同(tong)一(yi)批(pi)號(hao)的(de)血(xue)清(qing)
，這(zhe)樣(yang)實(shi)驗(yan)條(tiao)件(jian)穩(wen)定(ding)，配(pei)液(ye)時(shi)調(tiao)整(zheng)pHzhijiaorongyi。meicipeiyeliangyishiyongliangzhouzuoyoudeliangweiyi，yicipeiyebuyaotaiduo，yishiduanqineiyongbuwanzaochengyingyangchengfensunshixuyaobuchong，ershiliangdayizaochengwuran。