fangwuranshejihexiaodumiejunxitongwuranshidaozhixibaopeiyangshibaideyigezhuyaoyinsu,yiner,eryanghuatanpeiyangxiangdezhizaoshangmenshejileduozhongbutongdezhuangzhiqujianshaohefangzhiwurandefasheng,qizhuyaotujingdoushijinliangjianshaoweishengwukeyishengchangdequyuhebiaomian,bingjiehezidongpaichuwuranzhuangzhilaiyouxiaofangzhiwurandechansheng。
例如,鑒於CO2培(pei)養(yang)箱(xiang)在(zai)使(shi)用(yong)過(guo)程(cheng)中(zhong)有(you)時(shi)會(hui)伴(ban)有(you)黴(mei)菌(jun)生(sheng)長(chang),為(wei)確(que)保(bao)培(pei)養(yang)箱(xiang)免(mian)受(shou)汙(wu)染(ran)並(bing)且(qie)保(bao)證(zheng)儀(yi)器(qi)箱(xiang)體(ti)內(nei)的(de)生(sheng)物(wu)清(qing)潔(jie)性(xing),相(xiang)繼(ji)問(wen)世(shi)了(le)多(duo)種(zhong)消(xiao)毒(du)滅(mie)菌(jun)方(fang)式(shi),如(ru)帶(dai)有(you)紫(zi)外(wai)消(xiao)毒(du)功(gong)能(neng)的(de)CO2培養箱(如HealForce品牌的HF151UV和HF212UV二氧化碳培養箱);還有的設計生產了HEPA過濾器能過濾培養箱內空氣,可過濾除去99.97%的0.3微米以上的顆粒(如HealForce品牌的HF160W二氧化碳培養箱);此外,還開發設計了能使箱內達到高溫濕熱的環境從而殺死汙染微生物,達到消毒滅菌目的的培養箱(如HealForce品牌的HF90和HF240二氧化碳培養箱)。
這些裝置對於細胞培養來說是必不可少,但選擇何種清潔裝置呢?首shou先xian,我wo們men考kao慮lv的de當dang然ran是shi各ge種zhong方fang式shi的de滅mie菌jun能neng力li,紫zi外wai消xiao毒du能neng力li是shi與yu紫zi外wai燈deng距ju離li目mu標biao的de距ju離li的de二er次ci方fang成cheng反fan比bi,距ju離li越yue遠yuan,消xiao毒du能neng力li越yue差cha,所suo以yi紫zi外wai消xiao毒du方fang式shi有you其qi局ju限xian性xing,難nan以yi達da到dao徹che底di滅mie菌jun的de要yao求qiu;HEPA過濾器由於受到過濾膜孔徑的影響,無法去除病毒和一些微小細菌,也有其局限性;相xiang比bi較jiao而er言yan,高gao溫wen消xiao毒du是shi目mu前qian比bi較jiao有you效xiao消xiao毒du滅mie菌jun的de方fang法fa,高gao溫wen消xiao毒du又you分fen為wei兩liang類lei,一yi是shi傳chuan統tong的de高gao溫wen幹gan熱re消xiao毒du,另ling一yi種zhong是shi先xian進jin的de高gao溫wen濕shi熱re滅mie菌jun。接jie下xia來lai我wo們men重zhong點dian說shuo一yi下xia高gao溫wen幹gan熱re和he高gao溫wen濕shi熱re兩liang種zhong方fang法fa的de優you劣lie。高gao溫wen濕shi熱re由you於yu蒸zheng汽qi潛qian熱re大da,穿chuan透tou力li強qiang,容rong易yi使shi蛋dan白bai質zhi變bian性xing或huo凝ning固gu,因yin此ci該gai法fa的de滅mie菌jun效xiao率lv比bi幹gan熱re滅mie菌jun法fa高gao。
其原因有三:①danbaizhininggusuoxudewenduyuqihanshuiliangyouguan,hanshuiliangyuda,fashengninggusuoxudewenduyudi。shiremiejundejuntidanbaizhixishoushuifen,suoyijiaotongyiwendudeganrekongqizhongyiyuninggu。②濕shi熱re滅mie菌jun過guo程cheng中zhong蒸zheng汽qi放fang出chu大da量liang潛qian熱re,加jia速su提ti高gao濕shi度du。因yin而er濕shi熱re滅mie菌jun比bi幹gan熱re所suo需xu溫wen度du低di,如ru在zai同tong一yi溫wen度du下xia,則ze濕shi熱re滅mie菌jun所suo需xu時shi間jian比bi幹gan熱re短duan。③shiredechuantoulibiganreda,shiqishenbuyenengdadaomiejunwendu,gushirebiganreshouxiaohaoyixie。suoyigaowenxiaodubingbushijiandandekanxiaoduwendu,zhuyaoshikanshifoushirexiaodu。
另外,從使用角度看,濕熱消毒一般控製在90°C就能達到很徹底的消毒效果,整個消毒過程中培養箱內的所有附件都不用取出,可以全部進行消毒;而幹熱消毒為了達到較好的效果,溫度一般都在100°C以上,在這種溫度下消毒培養箱內的傳感器、HEPA過濾器等都要在消毒過程中取出,等消毒結束再裝上,這樣即麻煩,附件又不能同時消毒,而且增加二次汙染的幾率,再者要達到100°C以(yi)上(shang)的(de)高(gao)溫(wen),培(pei)養(yang)箱(xiang)的(de)加(jia)熱(re)係(xi)統(tong)的(de)電(dian)熱(re)絲(si)必(bi)然(ran)要(yao)加(jia)粗(cu),這(zhe)會(hui)導(dao)致(zhi)培(pei)養(yang)箱(xiang)的(de)溫(wen)度(du)控(kong)製(zhi)難(nan)度(du)增(zeng)加(jia),均(jun)一(yi)性(xing)變(bian)差(cha)。所(suo)以(yi)我(wo)們(men)建(jian)議(yi)用(yong)戶(hu)在(zai)選(xuan)購(gou)二(er)氧(yang)化(hua)碳(tan)培(pei)養(yang)箱(xiang)時(shi)選(xuan)擇(ze)含(han)高(gao)溫(wen)濕(shi)熱(re)滅(mie)菌(jun)方(fang)式(shi)的(de)培(pei)養(yang)箱(xiang)。目(mu)前(qian)市(shi)麵(mian)上(shang)有(you)高(gao)溫(wen)濕(shi)熱(re)滅(mie)菌(jun)功(gong)能(neng)的(de)二(er)氧(yang)化(hua)碳(tan)培(pei)養(yang)箱(xiang)極(ji)少(shao),而(er)HealForce(力康)牌HF90氣套式二氧化碳培養箱能夠做到。HF90培養箱包含90°C高溫濕熱滅菌係統,同時配合其電解拋光不鏽鋼圓邊圓角內腔體設計、箱內冷凝水控製係統、CO2進氣口HEPA過濾、外門開關檢測係統、三扇內玻璃小門等防汙染設計,真真正正是一款理想的二氧化碳培養箱。
手機版








