1、取鏡安放

手shou持chi顯xian微wei鏡jing的de正zheng確que方fang法fa應ying是shi右you手shou握wo住zhu鏡jing臂bi
，左zuo手shou托tuo住zhu鏡jing座zuo。切qie不bu可ke單dan手shou斜xie提ti，以yi防fang目mu鏡jing滑hua出chu。使shi用yong顯xian微wei鏡jing觀guan察cha時shi，顯xian微wei鏡jing應ying放fang在zai身shen體ti前qian方fang略lve偏pian左zuo的de地di方fang，以yi便bian用yong左zuo眼yan觀guan察cha
，右you手shou繪hui圖tu。　

2、對光 

轉動轉換器
，使低倍物鏡對準通光孔。注意物鏡的前端與載物台要保持2厘(li)米(mi)的(de)距(ju)離(li)。雙(shuang)眼(yan)睜(zheng)開(kai)
，左(zuo)眼(yan)注(zhu)視(shi)目(mu)鏡(jing)，將(jiang)遮(zhe)光(guang)器(qi)上(shang)較(jiao)大(da)的(de)光(guang)圈(quan)對(dui)準(zhun)通(tong)光(guang)孔(kong)，轉(zhuan)動(dong)反(fan)光(guang)鏡(jing)，使(shi)光(guang)線(xian)通(tong)過(guo)通(tong)光(guang)孔(kong)反(fan)射(she)到(dao)鏡(jing)筒(tong)內(nei)。從(cong)目(mu)鏡(jing)中(zhong)就(jiu)可(ke)見(jian)到(dao)一(yi)個(ge)白(bai)亮(liang)的(de)圓(yuan)形(xing)視(shi)野(ye)。如(ru)光(guang)線(xian)過(guo)於(yu)強(qiang)烈(lie)
，可(ke)調(tiao)小(xiao)光(guang)圈(quan)或(huo)用(yong)平(ping)麵(mian)反(fan)光(guang)鏡(jing)。

3
、壓片

壓片是將切片、塗(tu)片(pian)或(huo)裝(zhuang)片(pian)等(deng)玻(bo)片(pian)標(biao)本(ben)用(yong)金(jin)屬(shu)壓(ya)片(pian)夾(jia)固(gu)定(ding)在(zai)載(zai)物(wu)台(tai)上(shang)。壓(ya)片(pian)時(shi)

，要(yao)使(shi)玻(bo)片(pian)上(shang)的(de)標(biao)本(ben)正(zheng)對(dui)通(tong)光(guang)孔(kong)的(de)中(zhong)心(xin)
，標(biao)本(ben)小(xiao)時(shi)尤(you)其(qi)注(zhu)意(yi)這(zhe)一(yi)點(dian)。否(fou)則(ze)
，標(biao)本(ben)會(hui)偏(pian)出(chu)視(shi)野(ye)
，調(tiao)焦(jiao)時(shi)找(zhao)不(bu)到(dao)標(biao)本(ben)。　

4、調焦觀察　　

用低倍物鏡觀guan察cha時shi，不bu論lun觀guan察cha何he種zhong玻bo片pian標biao本ben，首shou先xian應ying用yong低di倍bei物wu鏡jing觀guan察cha。當dang對dui好hao光guang後hou，把ba玻bo片pian標biao本ben放fang在zai載zai物wu台tai上shang，用yong壓ya片pian夾jia壓ya住zhu

，並bing使shi玻bo片pian標biao本ben中zhong的de標biao本ben正zheng對dui通tong光guang孔kong的de中zhong心xin。然ran後hou順shun時shi針zhen轉zhuan動dong粗cu準zhun焦jiao螺luo旋xuan
，使shi鏡jing筒tong緩huan緩huan下xia降jiang，直zhi到dao物wu鏡jing接jie近jin玻bo片pian標biao本ben為wei止zhi（一般距蓋玻片2―3mm）。jingtongxiajiangshi，yanjingxucongpangbianzhushiwujing
，yimianwujingpengdaobopianbiaoben，yasuigaibopian
，sunhuaijingtou。ranhouzuoyanxiangmujingneiguancha，tongshifanfangxiangzhuandongcuzhunjiaoluoxuan，shijingtonghuanhuanshangsheng，zhidaoduizhunjiaodian
，kanqingwuxiangweizhi。zaizhuandongxizhunjiaoluoxuan，laihuitiaojie
，shikandaodewuxianggengjiaqingxi。ruguowuxiangbuzaishiyezhongjian
，zekeyibianguancha，yibianyongshouyidongbopianbiaoben，zhidaosuoyaoguanchadewuxiangjinrushiyezhongjian。zhidezhuyideshi，congmujingzhongkandaodewuxiangshidaoxiang。yinci，bopianyidongdefangxiangyushiyeliwuxiangdeyidongfangxiangzhenghaoxiangfan。